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KMID : 0352219930150010025
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Kyung Hee Dental Journal
1993 Volume.15 No. 1 p.25 ~ p.36
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THE EFFECT OF RED GINSENG, GANODERMA LUCIDUM AND CYCLOPHOSPHAMIDE ON THE TRANSPLANTED TUMOR IN THE GINGIVA
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Abstract
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The purpose of this study was to evaluate the inhibitory effect of Red ginseng, Ganoderma lucidum and Cyclophosphamide on the oral carcinogenesis by the transplantation of Sarcoma 180 ascite tumor cells into the mandibular gingiva.
Three month-aged 56 mice(ICR), weighing 20gm were devided 4 experimental groups which group 1(12 mice) were transplanted 0.05ml Sarcoma 180 ascite tumor cells per a mouse into the mandibular gingiva with 25 Gauge syringe, group 2(12 mice) were supplied Red ginseng solution for drinking water and administered orally 1mI Ganoderma lucidum solution for 3 days interval, after transplantatin of Sarcoma 180, group 3(12 mice) were transplanted Sarcoma 180 after administered Red ginseng and Ganoderma lucidum for 2 weeks, group 4(18 mice) were transplanted Sarcoma 180 and injected Cyclophosphamide intoperitonium with a dosage of 25mg/Kg for 3 days interval that was dissolved in distilled water, after administered Red ginseng and Ganoderma lucidum for 2 weeks, and three control groups which group 5(6 mice) administered Red ginseng and Ganoderma lucidum, group 6(6 mice) were injected Cyclophosphamide only, and group 7(2 mice) were untreated control.
Two mice of group 1, 2, 3, and 3 mice of group 4, and 1 mice of group 5, 6 were sacrificed at 4,6,8,10,12,14 days after experiment, the mandible was removed, fixed in 10% N-Forrnalin, and decalcified in 5% nitric acid. And embedded in paraffin, sectioned to 5-6μm thickness, and stained with Hematoxylin-Eosin and Van Gieson.
The results as follows
1. Sarcoma 180 trasplanted group revealed inflammatory reaction and necrosis of transplanted portion, located at 4 days, obvious malignancy of tumor cells at 6, 8 days, infiltrative proliferation into adjacent tissue and alveolar bone of apical portion through the alveolar bone and the periodontal membrane.
2. Tumor growth in the group which administered Red ginseng and Ganoderma lucidum after transplantation were slightly inhibited, and the group which transplanted tumor after administration of Red ginseng and Ganoderma lucidum for 2 weeks inhibited markedly than group 1.
3. Tumor growth in the group which transplantation and injection of Cyclophosphamide after administration of Red ginseng and Ganoderma lucidum obviously inhibited than group 2 and 3.
4. In control groups, the group which administrated Red ginseng and Ganoderma lucidum revealed vascular conjestion, the group which injected Cyclophosphamide revealed vascular congestion and slightly hemorrhagic feature.
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KEYWORD
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